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KMID : 0357119920140020287
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Korean Journal of Immunology
1992 Volume.14 No. 2 p.287 ~ p.296
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Effects of cAMP and cGMP on the Blockade of TCR-CD3-mediated Cytoplasmic Free Calcium Increases by Cholera Toxin in Human Peripheral Blood T lymphocytes
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신부안
류필열/이시은/장미영/임혁/이현철
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Abstract
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Perturbation of the TCR-CD3 complex by anti-CD3 antibodies resulted in the increases of the concentration of the cytoplasmic free calcium ([Ca2+]) in the human peripheral blood T lymphocytes and the malignant yhiman T-cell line Jurkat. In the
human
peripheral blood T lymphocytes, cholera toxin (CT) inhibited anti-CD3-induced calcium mobilization slightly. But cAMP an cGMP reduced the concentration of [Ca2+] markedly and the addition of anti-CD3 antibodies did not elicit any changes of
[Ca2+]
in
the cAMP or cGMP on the calcium mobilization were similar to that of cAMP or cGMP alone. Meanwhile, CT didd not effect on the expression of the CD3 molecule on the human peripheral blood T lymphocytes. But cAMP reduced the expression of the CD3
molecule
and the effects of the CT combined with cAMP or cGMP on the expression of the CD3 molecule were similar to that of cAMP or cGMP alone.
In Jurkat, CT inhibited anti-CD3-induced calcium mobilization. CAMP did not affect the blockade of the anti-CD3-induced calcium mobilization by CT. Meanwhile, CT reduced the expression of the CD3molecule on Jurkat and cAMP did not effects on
that.
CGMP
recovered the reduced expression of the CD3 molecule caused by CT.
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KEYWORD
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